Description
Quantity: 20 x 5 mL
Unit Size: Each
All members of Enterobacteriaceae are, by definition, glucose fermenters, resulting in the production of acid. MR-VP Broth is recommended for use in qualitative procedures for the performance of Methyl Red (MR) and Voges-Proskaur (VP) test as an aid in the identification of enteric gram-negative bacilli.
• Both MR and VP tests can be performed in the same medium.
• Easy-to-differentiate:MR positive organisms show a red color development and VP positive organisms produce a pink-red color development, while MR and VP negative organisms produce yellow color on the surface of the medium.
• Ready-to-use: Convenience of prepared media.The MR test is based on the use of a pH indicator, methyl red, to determine the hydrogen ion concentration present when an organism ferments glucose^4. This medium contains buffered peptone water, which supports growth of organisms. MR-positive organisms continue to produce acids, overcoming the phosphate buffer and resulting in a low pH and a red color development. MR-negative organisms further metabolize the initial fermentation products by decarboxylation to produce neutral acetyl methyl carbinol (acetoin), which results in a neutral pH.The VP test is based on the detection of neutral end-product acetylmethylcarbinol derived from glucose metabolism and is used primarily to separate Escherichia coli (VP negative) from the Klebsiella-Enterobacter group (VP positive). Acetylmethylcarbinol is a precursor to 2,3-butanediol, both of which are oxidized to diacetyl which is the reactant for the pink-red color produced in the VP test^5.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
