Thermo Fisher, R061954, OF Medium w/1% Mannitol

Name: OF Medium w/1% Mannitol
Brand: Thermo Fisher
SKU: R061954

Quantity: 20 x 4 mL, Unit Size: Each, Determine oxidative and fermentative metabolism of mannitol by gram-negative bacilli using Thermo Scientific™ Remel™ OF Medium w/1% Mannitol (Hugh-Liefson). Hugh and Leifson first reported that microorganisms which utilize carbohydrates oxidatively produce only a minimal amount of acid^1. Oxidative-Fermentative (OF) Medium was developed with a ratio of peptone to carbohydrate that minimized the formation of alkaline by-products and enhanced acid production b

Catalog Number: R061954

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TagsMicrobiological Media and Media Additives Prepared Microbiology Media

CollectionsThermo Fisher Microbiological Media & Media Additives Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 x 4 mL
Unit Size: Each
Typical fermentation media contain approximately 2% peptone and 1% carbohydrate. In media containing high concentrations of peptones, oxidative microorganisms produce alkaline by-products in quantities that neutralize the acid produced from carbohydrate metabolism.OF Medium is a semisolid medium with a low peptone to carbohydrate ratio that contains the pH indicator bromthymol blue. Organisms which metabolize carbohydrate in OF medium produce acid in quantities sufficient to prevent its neutralization by alkaline peptone by-products.
• Ready to use– Convenience of prepared media
• Uses 4 mL of 1% mannitol as carbohydrate source
• Easy to interpret– Color change from green to yellow indicates positive test for acid production.When testing for motility, growth diffused away from stab line of inoculation indicates a positive test whereas growth confined to stab line indicates negative test.Acid production in the open tube (without mineral oil overlay) indicates oxidative metabolism whereas acid production in both (with and without mineral oil overlay) tubes indicates fermentative metabolismOF Medium with Mannitol uses 4 mL of 1% mannitol as a fermentable carbohydrate. The medium contains casein peptone as the substrate and bromthymol blue as the pH indicator. Dipotassium phosphate promotes fermentation and also serves as a buffer. Agar allows for detection of motility and aids in even distribution of acid produced at the surface of the medium.

Specifications

General

Certifications/Compliance	Industrial Reference: BAM.
Description	OF Medium w/1% Mannitol (Hugh-Liefson)
Format	15mm x 103mm Tube
Quantity	20 x 4 mL
Product Type	Microbiology Media
Unit Size	Each

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Thermo Fisher, R061954, OF Medium w/1% Mannitol

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