Description
Quantity: 20 x 1 mL
Unit Size: Each
Identify and differentiate Enterobacteriaceae and other microorganisms on the basis of β-D-galactosidase activity with ONPG Broth.
• Ready to use– Convenience of prepared media
• Easy to interpret– Development of yellow color indicates a positive testThe fermentation of lactose depends on the action of two enzymes, permease and β-galactosidase. Permease allows lactose to enter the cell wall and β-galactosidase breaks down the lactose to glucose and galactose which can then be metabolized. In certain slow lactose-fermenting organisms, including Citrobacter spp., lactose enters the cells slowly due to permease deficiency^4. Unlike lactose, ONPG penetrates the bacterial cell in the absence of permease and is hydrolyzed by β-galactosidase releasing o-nitrophenol resulting in a yellow color.The medium contains peptone that provides nitrogenous compounds and amino acids essential for bacterial growth. Sodium chloride provides essential electrolytes and maintains osmotic equilibrium. ONPG acts as the substrate that is hydrolysed by β-galactosidase.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
