Thermo Fisher, R09556, Middlebrook 7H9 Broth w/Polysorbate 80

Quantity: 100 x 5 mL
Unit Size: Each
Use Middlebrook 7H9 Broth w/Polysorbate 80 for differentiation of Mycobacterium species based on their ability to reduce potassium tellurite.
• Ready-to-use–Convenience of prepared media.
• Easy-to-interpret –Based on tellurite reduction.Middlebrook 7H9 Broth w/Polysorbate 80 contains inorganic compounds that supply potassium, sulfur, magnesium, and phosphorus necessary for the growth of mycobacteria. Inorganic copper, iron, zinc, and calcium are growth stimulators. Sodium citrate holds certain inorganic cations in solutions. Ammonium sulfate is a source of nitrogen and dextrose is a source of carbon. Albumin binds to the free fatty acids which may be toxic to Mycobacterium species and acts as a protective agent. Catalase destroys toxic peroxides which may be present in the medium and catalyzes the reaction of iron with molecular oxygen. Biotin is required for carboxylation and decarboxylation reactions and pyridoxine is a precursor of enzymatic activity. Polysorbate 80 is a wetting agent that encourages more homogenous growth.