Thermo Fisher, R14207, Rhod-5N, Tripotassium Salt, cell impermeant

Quantity: 500 μg
Unit Size: Each
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca^2+. They have uses in many calcium signaling investigations, including measuring Ca^2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca^2+ release generated by photoreceptors and photoactivatable chelators.
• Fluorescence signal from these cells is generally measured using fluorescence microscopy
• Fluorescence intensity increase upon binding Ca^2+: >100 fold
• K_dfor Ca^2+in absence of Mg^2+, in buffer: approximately 320μM
• Exhibit fluorescence increase upon binding Ca^2+with little shift in wavelength
• In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn^2+, Zn^2+, Pb^2+) with substantially higher affinity than Ca^2+
• Perturbations to calcium measurements caused by presence of these ions can be controlled using heavy metal-selective chelator TPENCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling