Thermo Fisher, R211667, Nitrocefin Disk

Quantity: 25 Disks/Vial
Unit Size: Each
Since Abraham and Chain confirmed β-lactamase production by microorganisms to be responsible for antibiotic resistance, similar enzymes have been identified in various species. Some enzymes selectively hydrolyze penicillin class antibiotics (i.e., penicillinases), other enzymes hydrolyze all β-lactamase antibiotics except carbapenems (i.e. cephalosporinases) and some enzymes hydrolyze both^5.
• Ready to use- Nitrocefin impregnated disks.
• Rapid results
• Conforms to CLSI - Performance Standards for Antimicrobial Disk Susceptibility Tests – (Approved Standard M2-A8 and M11-A5)
• Easy to interpret- With color changeThe cephalosporin nitrocefin is the substrate used in this test. β-lactamase hydrolyzes the β-lactam ring of nitrocefin, producing cephalosporanic acid^6. This results in the pale yellow nitrocefin changing to a pink product upon hydrolysis^7. Aerobic and anaerobic β-lactamase-producing bacteria affect this color; organisms that fail to produce β-lactamase do not alter the pale-yellow color of nitrocefin within the time limits of test.