Thermo Fisher, R25001, Zeocin™ Selection Reagent

Name: Zeocin™ Selection Reagent
Brand: Thermo Fisher
SKU: R25001

Quantity: 8 x 1.25 mL, Unit Size: Each, Gibco Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. It acts by binding to and cleaving DNA, leading to cell death, and is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. Working concentration range is 50–400 μg/mL depending on the cell type. This product is supplied at 100 mg/mL in sterile water in 8 x 1.25 mL vials.

Catalog Number: R25001

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Category

Antibiotics & Antimycotics

Pack Size8 vials

TagsCell Culture Media Antibiotics Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 8 x 1.25 mL
Unit Size: Each
• Gibco™ Zeocin™ Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced byStreptomyces verticillus
• Zeocin™ Selection Antibiotic acts by binding to and cleaving DNA, leading to cell death
• It is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures
• Working concentration ranges between 50–400 μg/mL depending on the cell type
• This product is supplied at 100 mg/mL in sterile water, in 8 x 1.25 mL vials

Specifications

General

Concentration	100 mg/mL
Culture Type	Mammalian Cell Culture, Insect Cell Culture
For Use With (Application)	Eukaryotic Selection/Stable Cell Line Generation, Bacterial Selection
Product Line	Zeocin
Quantity	8 x 1.25 mL
Form	Liquid
Product Type	Antibiotic
Sterility	Sterile-filtered
Unit Size	Each

FAQs

Question: What concentration is supplied?

Answer:The reagent is supplied at 100 mg/mL in sterile water.

Question: How is it packaged?

Answer:This configuration contains eight 1.25 mL vials.

Question: What selection systems can use Zeocin?

Answer:It can be used with compatible mammalian, insect, yeast, bacterial, and plant cells.

Application Note

Zeocin™ Selection Reagent is a sterile solution of phleomycin D1 used for selection of cells carrying a compatible Zeocin-resistance gene. The antibiotic binds and cleaves DNA after activation inside susceptible cells, leading to cell death and allowing resistant clones to survive. This configuration is supplied at 100 mg/mL in sterile water as eight 1.25 mL vials. It can be used for selection in mammalian, insect, yeast, bacterial, and plant cell systems. Typical working concentrations fall within roughly 50–400 µg/mL, but the optimal level should be established empirically with a kill curve because sensitivity differs by cell type and culture condition. The divided-vial format reduces repeated freeze-thaw exposure of a larger stock. Store the reagent frozen at −10 to −30 °C and thaw only the amount needed for the current selection workflow.

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