Thermo Fisher, R37630, ReadyProbes™ Tissue Autofluorescence Quenching Kit

Quantity: 1 Kit
Unit Size: Each
• The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin.
• If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays.
• The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.
• The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio.
• Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature.
• This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.