Description
Quantity: 100 Tests/Kit
Unit Size: Each
The Chlamydia Culture Confirmation Kit uses fluorescein-conjugated murine monoclonal antibodies to stain inclusions and/or elementary bodies in cell monolayers 48 hours after inoculation of a clinical specimen. The fluorescein-labeled monoclonal antibodies recognize both the major outer membrane protein (MOMP) of all C. trachomatis serovars and the genus-specific lipopolysaccharide (LPS) of Chlamydia.When the antibody conjugate is added to a cover slip containing ethanol-fixed tissue culture cells, the reagent binds specifically to any chlamydial inclusions present in the cells. A washing step removes unbound antibody conjugate. When the stained coverslip is viewed under a fluorescence microscope, large bright apple-green inclusions within infected cells are observed against a red counterstained background of cells.
• Ready-to-use– Liquid reagents
• Convenient– Color coded dropper bottles
• Easy-to-interpret– Fixed, stained cultures that are positive for chlamydiae contain inclusion bodies that fluoresce bright green, against a cell monolayer counter-stained dark red. The presence of one inclusion body is considered positive.The Chlamydia Culture Confirmation Kit contains Chlamydia Confirmation Reagent and Mounting Fluid (R62230). Chlamydia Confirmation Reagent contains 5.5 mL of solution containing fluorescein-labeled purified murine monoclonal antibodies, Evans Blue counterstain, an inhibitor of nonspecific stain, and 0.098% sodium azide in a protein-stabilized buffer solution.The Mounting Fluid provides 5.0 mL of mounting fluid, that contains of buffered glycerol, with 0.01% sodium azide as preservative.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
