Thermo Fisher, R76207, Flp-In™-Jurkat Cell Line

Quantity: 1 mL
Unit Size: Each
• These cells contain single stably integrated FRT site at transcriptionally active genomic locus
• Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest
• Created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™-resistant clones
• Co-transfection of Flp-InCell Lines with Flp-Inexpression vector and Flp recombinase vector, pOG44, results in targeted integration of expression vector to same locus in every cell, ensuring homogeneous levels of gene expression
• Flp-In-Jurkat cell lines work well with vectors that express gene from CMV promoterConstitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration