Thermo Fisher, S7020, SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO

Name: SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
Brand: Thermo Fisher
SKU: S7020

Quantity: 250 μL, Unit Size: Each

Catalog Number: S7020

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Category

Fluorescent Dyes

Pack Size250 µL

TagsCell Analysis Products Cell Based Assays Cell Structure Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 250 μL
Unit Size: Each
SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells.Simple Viability Determination SYTOX Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, but will easily penetrate compromised membranes characteristic of dead cells. Because it exhibits >500-fold fluorescence enhancement upon binding nucleic acids, briefly incubating dead cells with SYTOX Green will result in bright green fluorescence with an emission peak of 523nm when excited by a 488nm argon-ion laser or any other 450–490nm source. The exceptional brightness of the signal produced in dead cells makes SYTOX Green particularly useful for determining viability of not just mammalian cells, but both Gram-positive and Gram-negative bacteria.Part of the Invitrogen Family of Cell Viability Stains and Assays SYTOX stains for dead cells are available in a variety of colors, including red, blue, and orange. SYTOX stains have been incorporated into a number of assays for apoptosis, cell viability, and metabolism. 5mM solution in DMSO.
• Impermeant to live cells
• Excitation/Emission: 504/523nm
• Use with 488 Argon-ion laser
• Works with mammalian cells and both Gram-positive and Gram-negative bacteriaCell Adhesion, Cell Analysis, Cell Cycle, Cell Structure, Cell Tracing and Tracking, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Cell Cycle Assays, Flow Cytometry Staining by Cell Structure, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, Immunofluorescence (IF), Immunofluorescence Counterstaining, Mounting and Fade Prevention, Immunofluorescence Staining and Detection, Microbial Tracking, Nucleus, Nucleoli and Nuclear Envelope, Organelle Tracing

Specifications

General

Color	Green
Detection Method	Fluorescence
Dye Type	Cell-Permeant
Emission	523 nm
Excitation Wavelength Range	504 nm
For Use With (Equipment)	Flow Cytometer
Form	Solution
Format	Tube(s)
Product Line	SYTOX
Quantity	250 μL
Shipping Condition	Room Temperature
Volume (Metric)	250 μL
Label Type	Fluorescent Dye
Product Type	Nucleic Acid Stain
SubCellular Localization	Nucleus
Unit Size	Each

FAQs

Question: What concentration and volume are supplied?

Answer:The product contains 250 µL of a 5 mM solution in DMSO.

Question: What are the fluorescence maxima?

Answer:Excitation and emission maxima are approximately 504 nm and 523 nm.

Question: What cells does the dye preferentially label?

Answer:It preferentially labels cells with compromised plasma membranes, making it useful as a dead-cell indicator.

Application Note

SYTOX™ Green is a high-affinity nucleic-acid stain that enters cells with compromised plasma membranes while remaining largely excluded from viable cells. This configuration contains 250 µL of a 5 mM solution in DMSO. After binding nucleic acids, the dye produces intense green fluorescence with excitation and emission maxima near 504/523 nm and is compatible with common 488 nm excitation systems. The reagent can be used as a dead-cell indicator in fluorescence microscopy, flow cytometry, fluorometry, and microplate-based viability measurements. One vial can support more than 1,500 labelings in a 96-well plate format under the standard procedure. Store the reagent at or below −20 °C, keep the vial upright, and protect it from light.

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