Thermo Fisher, S7563, SYBR™ Green I Nucleic Acid Gel Stain - 10,000X concentrate in DMSO, 500 μL

Name: SYBR™ Green I Nucleic Acid Gel Stain - 10,000X concentrate in DMSO, 500 μL
Brand: Thermo Fisher
SKU: S7563

Quantity: 500 μL, Unit Size: Each

Catalog Number: S7563

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Category

Nucleic Acid Stains

Pack Size500 µL

TagsGel Electrophoresis Equipment and Supplies Gel Electrophoresis Reagents and Buffers Electrophoresis Gel Stains

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 μL
Unit Size: Each
SYBR Green I offers greater sensitivity for dsDNA, making it especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR Green I stain is ideal for use with laser scanners.Product use 1mL stains 100 minigels.
• Sensitivity: at least four times greater than ethidium bromide for DNA in agarose gels
• Ease of use: gels soaked in diluted stain can be visualized without desalting
• Compatibility: with UV transilluminators, gel documentation systems, and laser scanners
• Flexibility: for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detectionAgarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

Specifications

General

Detection Location	In-Gel Detection
Detection Method	Fluorescence
Quantity	500 μL
Shipping Condition	Room Temperature
Target Molecule	DNA
Label or Dye	SYBR Green I
Product Type	Nucleic Acid Gel Stain
Unit Size	Each

FAQs

Question: What type of nucleic acid is this stain primarily used to detect?

Answer:It is highly sensitive for fluorescent detection of double-stranded DNA in gels.

Question: At what concentration is the stock supplied?

Answer:The product is supplied as a 10,000X concentrate in DMSO.

Question: How much stain is supplied?

Answer:This configuration contains 500 µL.

Application Note

SYBR™ Green I Nucleic Acid Gel Stain is used for highly sensitive fluorescent detection of double-stranded DNA in agarose and polyacrylamide gels. The dye binds nucleic acids and produces strong green fluorescence under suitable illumination, enabling visualization of DNA bands at lower concentrations than many traditional intercalating stains. This configuration contains 500 µL of a 10,000X concentrate in DMSO. The stain supports in-gel detection and can be incorporated into compatible staining workflows after electrophoresis. Because the concentrate is light sensitive, it should be stored at -20°C protected from light and moisture. The high-concentration format allows many staining runs from a small stock volume and is useful for molecular-biology applications that require sensitive detection of PCR products, restriction fragments and other double-stranded DNA samples.

Ordering

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