Thermo Fisher, SD0011, Lambda DNA

Name: Lambda DNA
Brand: Thermo Fisher
SKU: SD0011

Quantity: 500 μg, Unit Size: 500 µg, Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles.

Catalog Number: SD0011

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General

Concentration	0.3 μg/μL
Final Product Type	DNA
For Use With (Application)	Cloning/Nucleic Acid Gel Electrophoresis
Includes	[other]
Product Type	Lambda DNA
Quantity	500 μg
Sample Type	DNA
Species	Lambda phage
Unit Size	500 µg

FAQs

Question: What is the length of lambda DNA?

Answer:The DNA molecule is 48,502 base pairs long.

Question: What quantity and concentration are supplied?

Answer:This configuration contains 500 µg at 0.3 µg/µL.

Question: What are common applications?

Answer:It is commonly used for restriction-enzyme assays, preparation of DNA size standards, cloning, and gel electrophoresis.

Application Note

Lambda DNA is purified linear double-stranded DNA from bacteriophage lambda and is widely used as a defined substrate in molecular-biology workflows. This configuration contains 500 µg at a concentration of 0.3 µg/µL. The molecule is 48,502 base pairs long and has 12-base complementary single-stranded 5′ ends. It is useful for restriction-enzyme activity and specificity assays, preparation of DNA molecular-weight standards, cloning, and gel-electrophoresis demonstrations. The material is derived from a heat-inducible lysogenic E. coli W3110 strain carrying the cI857 Sam7 lambda genotype. Because the sequence and common restriction maps are well characterized, lambda DNA provides a convenient reference substrate for comparing digestion patterns across enzymes or experimental conditions. Store the DNA at approximately −20 °C in its supplied Tris-EDTA buffer.

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