Thermo Fisher, SD0021, Lambda DNA (dam–, dcm–)

Name: Lambda DNA (dam–, dcm–)
Brand: Thermo Fisher
SKU: SD0021

Quantity: 500 μg, Unit Size: 500 µg, Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles.

Catalog Number: SD0021

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Category

Blocking Reagents

Pack Size500 µg

TagsBiochemical Reagents DNA Reagents DNA Cloning and Mapping and Mutagenesis

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 μg
Unit Size: 500 µg
HighlightsPhage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is often used. The DNA sequence used to construct a phage alpha restriction map includes these mutations. The map shows enzymes that cut lambda DNA. Enzymes produced by Thermo Scientific are shown in orange (see Figure bellow).Lambda DNA (dam-, dcm-) is isolated from a heat-inducible lysogenic E. coli GM2163 strain.

Specifications

General

Concentration	0.3 μg/μL
Final Product Type	DNA
For Use With (Application)	CloningNucleic Acid Gel Electrophoresis
Includes	[other]
Product Type	Lambda DNA
Quantity	500 μg
Sample Type	DNA
Species	Lambda phage
Unit Size	500 µg

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Thermo Fisher, SD0021, Lambda DNA (dam–, dcm–)

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