Thermo Fisher, SM0244, GeneRuler Ready-to-Use DNA Ladders

GeneRuler Ready-to-Use DNA Ladders is used for electrophoretic sizing and approximate quantitation of short double-stranded DNA fragments. This configuration is the ready-to-use 100 bp ladder, providing 10 defined marker bands ranging from 100–1,000 bp and enough reagent for approximately 500 applications. The package is supplied as five 50 μg portions with loading dye already incorporated. It supports direct application to agarose gels and provides closely spaced bands for comparing PCR amplicons and restriction products in the shorter DNA-fragment range. The defined marker composition can also support approximate DNA quantity estimation when band intensity is compared under the same staining and imaging conditions. Its loading formulation contains tracking dyes, including xylene cyanol FF, bromophenol blue and orange G, which show the progress of electrophoresis before imaging. The ready-to-load preparation reduces handling compared with mixing a ladder and separate loading buffer for each run. The reagent is shipped at ambient temperature and has a specified shelf life of up to 24 months when stored at room temperature. Unlike the 1 kb ladder, this version does not extend to 10 kb and should be selected for smaller DNA fragments.