Thermo Fisher, SM0313, GeneRuler Ready-to-Use DNA Ladders

GeneRuler Ready-to-Use DNA Ladders is used for sizing and approximate quantitation of double-stranded DNA fragments during agarose-gel electrophoresis. This version is the ready-to-load 1 kb ladder with 14 DNA fragments spanning 250–10,000 bp and enough material for approximately 500 applications. The ladder is supplied as five 50 μg portions and includes colored loading components so it can be pipetted into a gel well without separately preparing loading dye. Clearly spaced reference bands support rapid orientation and fragment-size estimates when comparing PCR products, restriction digests or other DNA samples against the marker. The defined fragment masses also allow approximate assessment of DNA quantity from band intensity under consistent staining and imaging conditions. The reagent contains a multicolor tracking-dye system incorporating xylene cyanol FF, bromophenol blue and orange G for monitoring electrophoresis progression. It is compatible with standard agarose gels and retains a specified room-temperature shelf life of up to 24 months. The 250–10,000 bp 1 kb format is distinct from a 100 bp ladder, which targets a shorter fragment-size range.