Thermo Fisher, V23201, Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry

Name: Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry
Brand: Thermo Fisher
SKU: V23201

Quantity: 1 kit, Unit Size: 1 kit

Catalog Number: V23201

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Category

Apoptosis Detection Kits

TagsCell Analysis Products Cell Based Assays Apoptosis Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 kit
Unit Size: 1 kit
This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.

Specifications

General

Excitation/Emission	YO-PRO™-1: 491/509 cm, Hoechst 33342: 350/461 cm, PI: 535/617 cm
Flow Cytometer Laser Lines	488 nm
For Use With (Application)	Flow Cytometry
For Use With (Equipment)	Flow Cytometer
Form	Solution
No. of Reactions	200 Reactions
Product Line	Vybrant, YO-PRO
Product Type	Dead Cell Apoptosis Kit
Quantity	1 kit
Shipping Condition	Room Temperature
Conjugate	Hoechst 33342, Propidium Iodide, YO-PRO-1
Format	Kit
Unit Size	1 kit

Ordering

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