Waters, 176003068, ACQUITY UPLC Peptide CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 3/pk

Name: ACQUITY UPLC Peptide CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 3/pk
Brand: Waters
SKU: 176003068

Catalog Number: 176003068

Pricing

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Category

HILIC Columns

Pack Size3/pk

CollectionsWaters Service Parts & Kits for ACQUITY, Alliance & Arc Waters Sample Preparation: SPE, Syringe Filters & QuEChERS Waters Standards & Reagents for LC/MS, HPLC & GC

Description

Waters ACQUITY UPLC CSH130 C18 1.7 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. This kit consists of three ACQUITY UPLC CSH130 C18 columns packed with three different batches of sorbent and and one vial of Cytochrome C Digestion Standard (P/N 186006371). The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.

Specifications

General

Chemistry	C18
Separation Mode	Reversed Phase
Particle Substrate	Hybrid
pH Range Min	1 pH
pH Range Max	11 pH
Endcapped	Yes
Silanol Activity	Low
Molecular Weight Range Min	1000
Molecular Weight Range Max	15000
Particle Shape	Spherical
Particle Size	1.7 µm
Endfitting Type	Parker-style
Pore Size	130 Å
QC Tested	Peptide
Format	Method Validation Kit
Surface Area	185
System	UPLC, UHPLC
Particle Technology	CSH
USP Classification	L1
Inner Diameter	2.1 mm
Length	150 mm
Carbon Load	15 %
UNSPSC	41115709
Application	Peptide
Brand	ACQUITY UPLC
Product Type	Column Kits
Units per Package	3 pk

FAQs

Question: Why does the kit contain three separate columns?

Answer:The columns use three different sorbent batches to evaluate method robustness against batch-to-batch packing variation.

Question: Is a peptide test standard included?

Answer:Yes. One vial of Cytochrome C Digestion Standard is included for initial chromatographic benchmarking.

Question: Which peptides and column dimensions does the kit cover?

Answer:The intended molecular-weight range is approximately 1–15 kDa; each column has a 2.1 mm inner diameter, a 150 mm length and 1.7 µm, 130 Å CSH C18 particles.

Application Note

Waters ACQUITY UPLC Peptide CSH C18 Method Validation Kit supports peptide method validation with three 2.1 × 150 mm columns packed using three different batches of 1.7 µm, 130 Å charged-surface hybrid C18 sorbent. The low level of positive surface charge helps control peak shape for peptides in formic acid- or trifluoroacetic acid-based mobile phases. The kit also includes a Cytochrome C Digestion Standard vial for initial performance benchmarking. Designed for peptides of approximately 1–15 kDa, the columns support reversed-phase UPLC and LC-MS method robustness assessments.

Ordering

Notice

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