Waters, 186002011, Atlantis Silica HILIC Column, 100Å, 3 µm, 2.1 mm X 50 mm, 1/pk
Catalog Number: 186002011
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| Chemistry | HILIC |
|---|---|
| Separation Mode | Hydrophilic Interaction (HILIC) |
| Particle Substrate | Silica |
| pH Range Min | 1 pH |
| pH Range Max | 5 pH |
| Maximum Pressure | 6000 psi (415 Bar) |
| Endcapped | No |
| Silanol Activity | High |
| Particle Shape | Spherical |
| Particle Size | 3 µm |
| Endfitting Type | Waters |
| Pore Size | 100 Å |
| Format | Column |
| Surface Area | 330 |
| System | HPLC |
| Particle Technology | Silica |
| USP Classification | L3 |
| Inner Diameter | 2.1 mm |
| Length | 50 mm |
| UNSPSC | 41115709 |
| Brand | Atlantis |
| Product Type | Columns |
| Units per Package | 1 pk |
Answer:Highly polar compounds that retain poorly on reversed-phase columns are common candidates for this silica-based HILIC chemistry.
Answer:The column is 2.1 × 50 mm and contains 3 µm silica particles with 100 Å pores.
Answer:No. HILIC commonly uses mobile phases rich in organic solvent, with appropriate buffer content and sufficient re-equilibration.
Waters Atlantis Silica HILIC is a 2.1 × 50 mm HPLC column packed with 3 µm silica particles with 100 Å pores. Its hydrophilic interaction chromatography mechanism retains highly polar analytes that show insufficient retention on conventional reversed-phase columns. High-organic mobile phases, commonly rich in acetonitrile, enable the characteristic HILIC retention mechanism and provide orthogonal selectivity during method development. The short, narrow-bore format is useful when conserving solvent and developing fast methods for polar compounds, provided that system dispersion and equilibration are controlled. Waters specifies a pH range of 1–5 and a maximum operating pressure of about 415 bar. This is a bare-silica HILIC column, not a bonded C18 column, and it requires HILIC-appropriate mobile phase conditions.
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