Waters, 186005225, ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 150 mm, 1/pk

Based upon the highly successful BEH particle technology, the  ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 150 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note:  The same column is also available with the BEH200 SEC Protein Standard at no additional cost. (PN: 176003904)  Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Waters is phasing out Certificate of Analysis (COAs) for eCords. All column documents are now available at www.waters.com/coa

ACQUITY UPLC Protein BEH SEC Column is used for high-efficiency size-exclusion analysis of proteins, aggregates, and related biotherapeutic species. This column is 4.6 mm × 150 mm and contains 1.7 µm BEH particles with 200 Å pores. The sub-2-µm packing is optimized for protein SEC across an approximate molecular-weight range of 10,000 to 450,000 Da, supporting rapid measurement of monomer, aggregate, and fragment populations. The 4.6 mm format is designed for UPLC SEC systems with appropriately low extra-column volume, allowing higher efficiency and shorter analysis times than traditional HPLC SEC methods. It is suited to monoclonal antibodies, recombinant proteins, and other biotherapeutics where aggregate quantitation is important for development or quality control. The column can be operated with buffered aqueous mobile phases containing salt to minimize unwanted secondary interactions during molecular-size separation.