Merck, 05066-500G, Agarose

Name: Agarose
Brand: Merck
SKU: 05066-500G

Size:500 g, 3, 6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE, High EEO

Available Catalog Numbers

05066-500G 05066-50G

Catalog Number: 05066-500G

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Category

Agarose

Pack Size500 g

TagsAgarose Chemistry & Biochemicals Biochemicals

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.
Biochem/physiol Actions
Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.
Packaging
Packaging
50, 500 g in poly bottle
Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Specifications

General

UNSPSC Code	41105317
PubChem Substance ID	24845663
EC Number	232-731-8
NACRES	NA.25
MDL number	MFCD00081294
SMILES string	O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI	1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key	MJQHZNBUODTQTK-WKGBVCLCSA-N
grade	Molecular Biology
form	powder
impurities	DNases, none detected
ign. residue	≤1%
loss	≤10% loss on drying
EEO	0.23-0.27
transition temp	gel point 34-37 °C (1.5% solution)
gel strength	≥1500 g/cm2
anion traces	chloride (Cl-): ≤0.3%
cation traces	Ca: ≤0.05%
Quality Level	100

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Merck, 05066-500G, Agarose

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