Merck, 11191-100ML, Histopaque®-1119

General description
Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a relative density of 1.119 g/mL and pH 8.8–9.0.
Application
Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow
Biochem/physiol Actions
The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.
Features and Benefits
Histopaque®-1119 offers numerous advantages, such as:Optimal recovery of viable cells
Permits selective separation of blood cell types
Prevents cell distortion
Preserves cell viability
Minimal extraneous cell interference
Reproducible performance from batch to batch
Sterile-filtered and endotoxin-tested
Stable at 2-8°C for at least 2 years when protected from light

Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany