Merck, 70584-3, BugBuster® Protein Extraction Reagent

Name: BugBuster® Protein Extraction Reagent
Brand: Merck
SKU: 70584-3

Size:100 mL, Protein Extraction Reagent, Protein Extraction Solution

Available Catalog Numbers

70584-1000ML 70584-3 70584-4

Catalog Number: 70584-3

Pricing

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Category

Cell Lysis Buffers

Pack Size100 mL

TagsProtein Biology Protein Sample Prep Whole Cell Lysis Reagents & Enhancers

CollectionsMerck Protein Sample Prep: Complete Workflow Solutions Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.
Application
BugBuster® Protein Extraction Reagent has been used:
to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification
as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence
in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)

Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)

Specifications

General

NACRES	NA.32
UNSPSC Code	41106516
form	liquid
manufacturer/tradename	Novagen®
storage condition	OK to freeze
storage temp.	10-30°C
Quality Level	100

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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