Merck, 70584-4, BugBuster® Protein Extraction Reagent

General description
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.
Application
BugBuster® Protein Extraction Reagent has been used:
to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification
as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence
in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)

Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)