Merck, 82890-10MG, Pyridoxamine-5′-phosphate

General description
Pyridoxamine is a derivative of vitamin B₆. Pyridoxamine is a water-soluble vitamin. Vitamin B₆ derivatives contain a core pyridine ring. Pyridoxaminecontains an amino methyl group at pyridine′s 4′ position.
Application
Pyridoxamine-5′-phosphate is suitable:
in the enzymatic synthesis of cytidine diphosphate-4-keto-3, 6-dideoxyglucose
as a substrate for pyridoxamine-5′-phosphate phosphatase activity assay
as a substrate to characterize pyridoxine-5′-phosphate oxidase
for the absorbance and fluorescence studies of properties of NaBH4-reduced glycogen phosphorylase b to probe the microenvironment of PMP residue
as a substrate for characterization of pyridoxamine-phosphate transaminase(s) from various species and tissues

Biochem/physiol Actions
pK of pyridoxamine-5′-phosphate (pyridoxamine 5-phosphate, PAMP) in the singlet excited state has been evaluated by absorption and fluorescence spectral studies.
Pyridoxal 5′-phosphate can be converted to pyridoxamine-5′-phosphate in the presence of (S)-α-methylbenzylamine (MBA) as the amine donor by using pyruvate transaminase as a biocatalyst. Pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate are oxidized to pyridoxal 5′-phosphate (PLP) using flavin mononucleotide (FMN) as the immediate electron acceptor and oxygen as the ultimate electron acceptor by the E. coli enzyme pyridoxine (pyridoxamine) 5′-phosphate oxidase.