Merck, 94740-1G, Vanadyl ribonucleoside complexes

General description
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Application
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Biochem/physiol Actions
The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.