Description
General description
Molecular weight: 32.5-33 kDa
pI: 4.85
Application
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Biochem/physiol Actions
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Physical form
Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.