Merck, B2643-10MG, Biotinamidohexanoic acid N-hydroxysuccinimide ester

Application
Biotinamidohexanoic acid N-hydroxysuccinimide ester has been used in the biotinylation of antibodies.
Biochem/physiol Actions
Biotin is a label widely used in several biochemical applications and has a high affinity for avidin. A spacer chain is required to reduce steric hindrance while coupling biotin (a small molecule) to biopolymers (large molecules). Biotinamidohexanoic acid N-hydroxysuccinimide ester incorporates a 7-atom spacer with the N-succinimide leaving group. It can react with a primary amine in the pH range of 6.5-8.5. This biotinylation reagent incorporates an aminocaproyl spacer and thus can reduce the steric hindrance in binding avidin to some biotinylated compounds.
Disclaimer
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