ApplicationReagent for spectrophotometric determination of selenium.
3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
Biochem/physiol ActionsPeroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.
3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.
Preparation NoteDissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.
Other Notes2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research