Description
General description
JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Application
JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
insulin-enterotoxin ricin fusion gene (INS-RTB)
endothelial cells DNA derived from reverse transcribed RNA
leg genomic DNA from cricket flies
mitochondrial gene by conventional PCR
JumpStart™ REDTaq® DNA Polymerase is also suitable for DNA methylation analysis.
Features and Benefits
Reduces non-specific amplification
Increased target yield and specificity
Higher the amplification irrespective of the target concentration
Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
Assembled PCR reactions can be placed at room temperature for up to 2 hours
Packaging
The enzyme is provided with an optimized 10× reaction buffer.
Other Notes
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
