General descriptionβ-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about
Drug Metabolite AnalysisApplicationNew Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
- Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
- Removal of glucuronide conjugates such as for the preparation of Baicalein.
Biochem/physiol ActionsUsed for the hydrolysis of glucuronide conjugates in urinary
metabolite analysisEndogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Features and Benefits- pH optima 4.0 - 5.0.
- Sulfatase pH optima 6.2
- Low Sulfatase activity (<7,500 units/mL).
- Ammonium Sulfate suspension.
- Mollusk-derived beta-glucuronidase.
- Useful for acidic hydrolysis of glucuronides in low pH samples.
Physical formAqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Analysis NoteMany β-glucuronidases derived from mollusks also contain sulfatase activity.
Other NotesOne Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Similar to G0876, but produced by Sigma.
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
