Merck, G7017-5ML, β-Glucuronidase from Helix pomatia

General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

• Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
• Removal of glucuronide conjugates such as for the preparation of Baicalein.­

Biochem/physiol Actions
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Features and Benefits

• pH optima 4.0 - 5.0.
• Sulfatase pH optima 6.2
• Low Sulfatase activity (<7,500 units/mL).
• Ammonium Sulfate suspension.
• Mollusk-derived beta-glucuronidase.
• Useful for acidic hydrolysis of glucuronides in low pH samples.

Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Similar to G0876, but produced by Sigma.
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.