General descriptionDetermined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits- Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect medium.
- MabSelect Xtra uses the same recombinant protein A ligand as MabSelect
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.
Preparation NotePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis NoteTo view the Certificate of Analysis for this product, please visit
www.cytiva.com.
Other NotesThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal InformationHiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva