Description
General description
ProbeQuant™ G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides.
ProbeQuant™ G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex® G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μ to 50 μ.
Features and Benefits
For rapid purification of labeled DNA (20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography
Convenient: Prepacked with Sephadex® G-50 DNA Grade (see illustra™ Sephadex® G-50 and G-100 DNA Grade) and pre-equilibrated in STE containing 0.15% Kathon CG/ICP Biocide
Ready to use: Requires less than 4 min from sample application to collection of purified product
Designed for use in a microcentrifuge
Tested in nickase, single and double-stranded exonuclease and RNase assays
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
illustra is a trademark of Cytiva
