Description
General description
Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μ or agarose gel slices up to 900 mg.
illustra™ GFX™ PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μ or agarose gel slices up to 900 mg. The kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed.
illustra™ GFX™ PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX™ columns, collection tubes, color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water), and an instruction booklet.
Features and Benefits
For the isolation and concentration of DNA fragments (50 bp to 40 kb) from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
Fast and easy-to-use method with less than 10 min hands-on time.
Flexible 10 to 50 μ elution volume for different DNA concentration needs.
Improved column design with increased maximum volume, holding of up to 800 μ liquid volume (950 μ without a cap).
Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.
Addition of a visual color indicator to the capture buffer ensures optimal pH for maximum DNA binding and recovery.
Two choices of elution buffer provided to fit downstream applications.
Color-coded caps for ease of use.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
GFX is a trademark of Cytiva
illustra is a trademark of Cytiva
