Description
General description
Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-βGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
Dextrin Sepharose™ High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).
Application
Dextrin Sepharose™ High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose™ High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.
Features and Benefits
Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
Physiological conditions and mild elution preserve target Protein activity
Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva