Description
General description
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
Application
HiScreen™ Capto™ Blue is prepacked with the base matrix Capto™ to which Cibacron Blue 3G is covalently bound via a hydrophilic spacer immobilized with a stable amine bond. Capto™ Blue is highly chemically stable and has a more rigid Agarose base matrix than Blue Sepharose™ 6 Fast Flow. This allows the use of faster flow rates and larger sample volumes, leading to higher throughput and improved process economy. The application area is the same as for Blue Sepharose™ Fast FLow (e.g., purification of albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons, and related Proteins). The main benefit of Capto™ Blue compared to Blue Sepharose™ 6 Fast FLow is its improved chemical stability provided with a new coupling chemistry involving a spacer arm. It tolerates repeated cleaning-in-place and sanitization at both high and Low pH as well as autoclaving. Also the leakage of Cibacron Blue is reduced as compared to Blue Sepharose™ 6 Fast Flow.
Features and Benefits
Prepacked with Capto™ Blue
Excellent for method optimization and parameter screening due to the 10 cm bed height
Easily connected in series to achieve 20 cm bed height
Small bed volume for fast results and minimal sample/buffer consumption
Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
