Description
General description
HiPrep 16/10 Phenyl HP is a prepacked column designed for preparative hydrophobic interaction chromatography (HIC) with good resolution. Phenyl Sepharose HP chromatography resin is based on a matrix of highly cross-linked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic resin with no ionic properties. High mechanical strength of the matrix allows for high flow rates, together with good resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.
Features and Benefits
Aromatic HIC resin for intermediate purification and polishing.
Selectivity similar to Phenyl Sepharose 6 Fast Flow (Low Sub) but higher resolution due to the smaller 34 µm bead size.
Provides fast, easy high-capacity separations in a convenient format.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiPrep is a trademark of Cytiva