Description
Application
Isocitric dehydrogenase (IDH) has been used:for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
in isocitrate assay
Biochem/physiol Actions
Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.
Physical form
Solution in 50% glycerol in EDTA buffer salts, pH 6.0
Other Notes
One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.