Merck, L5006-50UN, Leucine Aminopeptidase, microsomal from porcine kidney

General description
Leucine Aminopeptidase belongs to the family of metalloprotease.
Application
Leucine Aminopeptidase, microsomal from porcine kidney has been used in the isolation of neprilysin (NEP) from rodent brain-C5 and for peptide digestion.
Biochem/physiol Actions
Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.
Physical form
Suspension in 3.5 M (NH₄)₂SO₄ solution, pH 7.7, containing 10 mM MgCl₂
Other Notes
One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.