Merck, S4942-5L, SYPRO™ Ruby Protein Gel Stain

General description
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:

• uses a simple staining protocol, with no possibility of overstaining
• delivers a linear quantitation range of over three orders of magnitude
• shows less protein-to-protein variability
• stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
• will not stain extraneous nucleic acids
• does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

Application
SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
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