Merck, T7572-250UN, Thrombin from human plasma

Name: Thrombin from human plasma
Brand: Merck
SKU: T7572-250UN

Size:250 units, Factor IIa, 400-1000 NIH units/mg protein

Available Catalog Numbers

T7572-1000UN T7572-250UN

Catalog Number: T7572-250UN

Pricing

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Category

Recombinant Growth Factors

Pack Size250 units

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Lyophilized powder containing sucrose, sodium chloride and Tris.
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an innovative flow cytometric approach for small-size platelet microparticles.
Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Specifications

General

UNSPSC Code	12352204
NACRES	NA.54
EC Number	232-648-7
MDL number	MFCD00082072
Enzyme Commission (EC) Number	3.4.21.5 ( BRENDA \| IUBMB )
form	lyophilized powder
specific activity	400-1000 NIH units/mg protein
impurities	HIV, hepatitis B and hepatitis C, tested negative
UniProt accession no.	P00734
storage temp.	−20°C
Quality Level	200
Gene Information	human ... F2(2147)

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Merck, T7572-250UN, Thrombin from human plasma

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