Thermo Fisher, 10359016, Bac-to-Bac™ Baculovirus Expression System

Name: Bac-to-Bac™ Baculovirus Expression System
Brand: Thermo Fisher
SKU: 10359016

Quantity: 1 kit, Unit Size: Each

Catalog Number: 10359016

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Category

Molecular Identification Kits

TagsMolecular Biology Reagents and Kits DNA Vectors

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 kit
Unit Size: Each
• Relies on generation of recombinant baculovirus by site-specific transposition inE. colirather than homologous recombination in insect cells
• Time-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10BacE. coli
• Competent Cells to form expression bacmid
• Bacmid is then transfected into insect cells for production of recombinant baculovirus particles
• Easy colony screening
• Parent bacmid in DH10BacE. colicontains segment oflacZα gene
• lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants
• Flexibility
• Comes with pFastBac1 vector that offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
• Two other vectors are offered separately
• pFastBacHT vector for production of histidine-tagged recombinant protein and pFastBacDual vector for expression of two proteins simultaneously
• Rapid purification of recombinant proteins
• Optional pFastBacHT vector produces histidine-tagged recombinant protein for rapid protein purification with nickel-chelating resinBaculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

General

Product Type	Baculovirus Expression System
Quantity	1 kit
Vector	pFastBac
Cloning Method	Restriction Enzyme/MCS
Product Line	Bac-to-Bac
Promoter	Polyhedrin
Protein Tag	Untagged
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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Thermo Fisher, 10359016, Bac-to-Bac™ Baculovirus Expression System

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General

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