Description
Quantity: 5000 Units
Unit Size: Each
The robust molecular structure of the RNaseOUT™ inhibitor together with a very high binding affinity for pancreatic-type RNases ensures superior protection of RNA from degradation making RNaseOUT™ highly effective and reliable in various molecular biology applications.Features
• Stable under prolonged reaction conditions—effective RNA protection from RNases at:4°C for at least 96 hours25°C for at least 96 hours37°C for 24 hours50°C for up to 6 hours
• Oxidation resistant—highly stable and requires low concentrations of DTT to retain RNase inhibiting activity
• Wide activity temperature range—maintains full activity from 4°C to 55°C
• Superior quality—stringent quality control and industry leading manufacturing processApplications
• In vitrotranscription and translation
• CRISPR guide RNA production
• RNA purification
• cDNA synthesis
• RT-PCR
• RT-LAMP
• RNA-seq
• RNA modification and labelingSourcePurified by affinity chromatography from E. coli expressing a cloned porcine gene.Performance and quality testing
• SDS-PAGE purity
• Endo-deoxyribonuclease assay
• Protein concentration
• Specific activity
• Performance evaluated by RT-PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate.Unit reaction conditions100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C
