Thermo Fisher, 15127, Pierce™ NeutrAvidin™ Coated Plates

Pierce™ NeutrAvidin™ Coated Plate 15127 is used to immobilize biotinylated antibodies, probes, peptides, and other biotin-containing conjugates for ELISA and target-specific binding assays. The clear 96-well polystyrene strip plate supports colorimetric detection and arrives coated with NeutrAvidin protein and preblocked with SuperBlock buffer. This ready-to-use surface avoids direct adsorption of the capture molecule to polystyrene, a process that can denature antibodies or other proteins. Biotin–NeutrAvidin binding instead provides gentle, affinity-based immobilization while preserving the protein component of a conjugate. NeutrAvidin is deglycosylated avidin with a near-neutral isoelectric point of 6.3, reducing nonspecific lectin binding and lowering nonspecific interactions in suitable assays. It also lacks the bacterial RYD sequence present in streptavidin, eliminating nonspecific binding associated with the RGD-binding domain of cellular adhesion receptors. These properties support low-background assays and improve capture of small, hydrophilic biotinylated molecules such as peptides that bind poorly to untreated polystyrene. The strip format allows selected wells to be used while unused strips remain stored, and one pack contains five plates. Store unopened pouches at 4 °C. After opening, return unused plates to a resealable bag with desiccant and keep them at 4 °C. A clear optical path, SuperBlock preblocking, and low-nonspecific-binding NeutrAvidin surface make this plate well suited to colorimetric biotin-capture immunoassays and probe-binding studies.