Description
Color: Clear
Format: Standard
Surface Treatment: Protein G
Unit Size: Each
Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.Highlights• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption• Orient antibodies for maximum antigen-binding capacity• Immobilize antibodies for plate assays without prior purification• Ensure minimal variation (<5% well-to-well) from consistent coating• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)Protein A/G Coated Plates• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G• Binds to most IgG species because it combines the specificities of Protein A and Protein G• Binds to Fc region of antibodies, ensuring optimal orientation• Binds well using a wide range of pHProtein A Coated Plates• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey• Binds strongly to mouse IgG2a, IgG2b and IgG3• Binds to Fc region of antibodies for optimal orientationProtein G Coated Plates• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat• Binds only to IgG—no cross-reactivity with other antibody classes• Binds to Fc region of antibodies for optimal orientation Protein L Coated Plates• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites• Binds to Fab fragments and ScFv• Does not bind bovine, goat or sheep immunoglobulin• Binds weakly to rabbit immunoglobulin• Pre-blocked to reduce nonspecific binding
