Description
Color: Clear
Format: Standard
Detection Method: Colorimetric
Unit Size: Each
Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.Features of Pierce Nickel Coated Plates include:• Ni^2+ activated surface enables metal-chelate binding of His-tagged proteins• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene• Better binding for sensitive assays compared to other commercially available nickel-activated plates• Detection limit: 1 ng of polyhistidine fusion protein• Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well• Activation level: 200 μLThese Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.
