Thermo Fisher, 15596026, TRIzol™ Reagent, 100 mL

Name: TRIzol™ Reagent, 100 mL
Brand: Thermo Fisher
SKU: 15596026

Quantity: 100 mL, Unit Size: Each

Catalog Number: 15596026

Pricing

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Category

Total RNA Isolation Kits

Pack Size100 mL

TagsMolecular Biology Reagents and Kits RNA Isolation and Purification Products

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mL
Unit Size: Each
TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.Key features of TRIzol™ Reagent include:• Permits the isolation of RNA, DNA, and protein from the same sample• Offers superior lysis capability, even with difficult sample types• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteriaReliably purify RNA from multiple sample volumes and sourcesTRIzol™ Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 10^6), as well as with large quantities of tissue (≥1 g) and cells (>10^7), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol™ Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol™ Reagent is free of protein and DNA contamination.Formulated for isolation of multiple molecular targetsTRIzol™ Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

Specifications

General

Elution Volume	20 to 600 μL
Final Product Type	Total RNA, DNA, Protein
For Use With (Application)	RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Purification Time	1 hr.
Quantity	100 mL
Shipping Condition	Room Temperature
Starting Material Amount	Bacteria: ≤10<sup>7</sup> cells<br />Cells: ≤10<sup>7</sup><br />Plant: ≤10<sup>7</sup> cells, 100 mg<br />Tissue: ≤100 mg<br />Yeast: ≤10<sup>7</sup> cells
Yield	DNA: ≤7 μg<br />RNA: ≤73 μg<br />Protein: varies
Isolation Technology	Organic Extraction
Sample Type	Plants
Unit Size	Each

FAQs

Question: What quantity is supplied?

Answer:This configuration contains 100 mL of TRIzol Reagent.

Question: What biomolecules can be recovered?

Answer:The workflow can isolate total RNA and can also support sequential recovery of DNA and protein from the same sample.

Question: What extraction chemistry is used?

Answer:The reagent uses a monophasic phenol and guanidine isothiocyanate formulation.

Application Note

TRIzol™ Reagent is a ready-to-use monophasic solution of phenol and guanidine isothiocyanate for isolation of high-quality total RNA and, when required, sequential recovery of DNA and protein from the same sample. This 100 mL format is suitable for cells and tissues from human, animal, plant, yeast, and bacterial sources. During extraction, the reagent rapidly disrupts cells and inactivates RNases, helping preserve RNA integrity. Chloroform partitioning separates the homogenate into an aqueous RNA phase and organic/interphase fractions containing DNA and protein, after which each target can be precipitated separately. The workflow can be completed in about one hour for routine samples and supports downstream applications including RT-PCR, qPCR, sequencing, microarray analysis, blotting, and other nucleic-acid or protein workflows. Store the reagent at 2–25 °C.

Ordering

Notice

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