Thermo Fisher, 18021014, Ribonuclease H, 30 U

Name: Ribonuclease H, 30 U
Brand: Thermo Fisher
SKU: 18021014

Quantity: 30 U, Unit Size: Each

Catalog Number: 18021014

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Category

Phosphatase Inhibitors

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 30 U
Unit Size: Each
• Source: Purified fromE. coliexpressing theE. coliRNase H gene on a plasmid
• Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
• Unit definition: One unit hydrolyzes 1nmol of RNA in^3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
• Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl_2, 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol^3H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°CRemoval of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

Specifications

General

Product Type	Ribonuclease H
Quantity	30 U
Shipping Condition	Approved for shipment on Wet or Dry Ice
Concentration	2 U/μL
Enzyme	RNase
Unit Size	Each

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Thermo Fisher, 18021014, Ribonuclease H, 30 U

Send an RFQ.

General

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