Description
Quantity: 20 Reactions
Unit Size: Each
The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents. The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency. Highlights:
• Non-radioactive– incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems
• Fast– RNA can be labeled in 0.5-2 hours with minimal downstream processing
• Easy to use– RNA ligase and optimized reaction buffer are included
• Economical– only a fraction of the cost of purchasing synthetic biotinylated RNA probes
• End-labeled– results in minimal disturbance of RNA secondary structure
• Flexible– label synthetic orin vitrotranscribed RNA probes ranging from 22 to 450 nucleotides
